The study examined the influence of store layout on the purchase behavior of consumers in Uyo metropolis. Specifically the study examined the influence of store design and layout on purchase behavior. A total of two hundred and forty four shoppers were sampled from five supermarkets in Uyo using the questionnaire as a data collection technique. Data collected was analyzed using different statistical processes such as descriptive statistics, multiple regression and Pearson product moment correlation. The study findings revealed that store layout and design significantly influenced purchase behavior. Its power of influence is significantly higher than other atmospheric factors like:- background music, ambient scent and colour. The author recommended that retailers should focus their resources on improving the particularly weak aspect of their layout and interior décor. Store atmospherics and layout design should fall in line with the store image.