Lecturers and staff are important resources in universities. Their work performance is closely related to work motivation. The motivation of lecturers and staff in the university is affected by many different factors. In this study, we focused on assessing the influence of 5 factors: income, co-worker relationship, development opportunities, working conditions and nature of work. The study was carried out on a sample of 163 subjects who are lecturers and staff of a public university in Vietnam. Methods of exploratory factor analysis EFA, linear regression analysis were used to test the research hypotheses. Research results showed that all 5 factors considered have a positive impact on the work motivation of lecturers and staff. In which, the income factor has the strongest influence. The results of this study provide data as a basis for building motivational strategies for lecturers and staff at universities in Vietnam.