NMR spectroscopy has a wide range of applications including exchange phenomena, the identification and structural studies of complex biomolecules. 1D 1H-NMR without water suppression, 1D Carbon, 1D 13C-DEPT135, 2D Cosy, 2D HSQC, 2D TOCSY, 2D HMQC, and 2D HMBC techniques were used to completely elucidate the structure of glucose with spectral induced at 400MHz.. The spectral were analysed using spinworks 3. The results obtained from the spectral data were systematically combined to elucidate the structure of the D-glucose. Full characterisation of D-glucose was achieved by assigning 1H and 13C signals, starting from the known to unknown signals.