Female Genital Mutilation (FGM) remains a pervasive cultural practice with weighty socio-cultural implications, particularly for adolescent females. This research provides insight into the nuanced socio-cultural impact of FGM on adolescent girls in Calabar, Cross River State, highlighting the multifaceted dimensions of this deeply ingrained practice. The study findings detail the complex web of socio-cultural factors contributing to the practice and continuance of FGM in Calabar, revealing the interplay between tradition, social norms, and the role of women in the community. Furthermore, this paper investigates the attitudes and perceptions of communities in the study area towards FGM, providing insights into the limitations and potential for cultural transformation. This study is significant to policymakers, healthcare professionals, and community leaders seeking to address the adverse effects of FGM on adolescent females in Calabar. By addressing the socio-cultural complexities surrounding FGM, this publication seeks to inform targeted interventions, promote community dialogue, and substantially contribute to the eradication of FGM, consequently improving the health status of adolescent females in Calabar and beyond.